abstract：:Goatpox disease and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are goatpox virus (GPV) and Echinococcus granulosus (E. granulosus), respectively. Both E. granulosus EG95 subunit vaccine and live-attenuated GPV AV41 vaccine have been widely used for prevention of both diseases in ...

journal_title：Journal of virological methods

authors： Liu F,Fan X,Li L,Ren W,Han X,Wu X,Wang Z

更新日期：2018-11-01 00:00:00

abstract：:In this study, we generated recombinant virus-like particles (VLPs) against family Filoviridae, genus Ebolavirus, species Zaire ebolavirus, strain Makona (EBOV) in Drosophila melanogaster Schneider 2 (S2) cells using the EBOV Makona. S2 cells were cotransfected with four viral plasmids encoding EBOV Makona proteins an...

journal_title：Journal of virological methods

authors： Park EM,Park SW,Lee YJ,Lee WJ,Choi W

更新日期：2018-11-01 00:00:00

abstract：:Healthcare workers (HCWs) are at significantly higher risk of exposure to influenza virus during seasonal epidemics and global pandemics. During the 2009 influenza pandemic, some healthcare organizations recommended that HCWs wear respiratory protection such as filtering facepiece respirators, while others indicated t...

journal_title：Journal of virological methods

authors： Blachere FM,Lindsley WG,McMillen CM,Beezhold DH,Fisher EM,Shaffer RE,Noti JD

更新日期：2018-10-01 00:00:00

abstract：:Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Accurate, rapid and simple detection of CDV is critical to improve disease management and prevent outbreaks. In this study, a visible and incubation instrument-fr...

journal_title：Journal of virological methods

authors： Wang J,Wang J,Li R,Shi R,Liu L,Yuan W

更新日期：2018-10-01 00:00:00

abstract：:Real-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A-E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of these agents is not yet available. Standardized TaqMan assays for detection of hepatitis viruses A-E have been described and applied...

journal_title：Journal of virological methods

authors： Granade TC,Kodani M,Wells SK,Youngpairoj AS,Masciotra S,Curtis KA,Kamili S,Owen SM

更新日期：2018-09-01 00:00:00

abstract：:Poxviruses are emerging pathogens in cetaceans, temporarily named 'Cetaceanpoxvirus' (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene ...

journal_title：Journal of virological methods

authors： Sacristán C,Catão-Dias JL,Ewbank AC,Ferreira-Machado E,Neves E,Santos-Neto EB,Azevedo A,Laison-Brito J Jr,De Castilho PV,Daura-Jorge FG,Simões-Lopes PC,Carballo M,García-Párraga D,Sánchez-Vizcaíno JM,Esperón F

更新日期：2018-09-01 00:00:00

abstract：:Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of MERS-CoV [Virol J. ...

journal_title：Journal of virological methods

authors： Shirato K,Semba S,El-Kafrawy SA,Hassan AM,Tolah AM,Takayama I,Kageyama T,Notomi T,Kamitani W,Matsuyama S,Azhar EI

更新日期：2018-08-01 00:00:00

abstract：:A newly developed integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) method and its applicability in UV disinfection studies is described. This method utilizes a cell culture system coupled with four RTqPCR assays to detect four species of human enterovirus (e.g., Enterovirus A, Enterovirus B,...

journal_title：Journal of virological methods

authors： Ryu H,Schrantz KA,Brinkman NE,Boczek LA

更新日期：2018-08-01 00:00:00

abstract：:Feline infectious peritonitis (FIP) is a fatal disease in cats worldwide. The aim of this study was to test two commercially available reaction mixtures in a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect feline Coronavirus (FCoV) in body cavity effusions of cats with and withou...

journal_title：Journal of virological methods

authors： Günther S,Felten S,Wess G,Hartmann K,Weber K

更新日期：2018-06-01 00:00:00

abstract：:Louping ill-like virus (LI) has been recently detected in two different locations in the north of Spain and separated by only around 400 km. Using molecular approaches, the viruses causing both outbreaks have been shown to be different to LI virus, but also different to each other. They have been called SSEV (Spanish ...

journal_title：Journal of virological methods

authors： Del Cerro A,Balseiro A,Casais R,Dalton KP,Salinas L,Saiz JC,Royo LJ

更新日期：2018-05-01 00:00:00

abstract：:Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was first reported in southern China in 2017. It can cause severe diarrhea disease in pigs. In order to detect this new emerging virus rapidly and reliably, a TaqMan-based real-time RT-PCR assay was established in this study. Specific pr...

journal_title：Journal of virological methods

authors： Zhou L,Sun Y,Wu JL,Mai KJ,Chen GH,Wu ZX,Bai Y,Li D,Zhou ZH,Cheng J,Wu RT,Zhang XB,Ma JY

更新日期：2018-05-01 00:00:00

abstract：:The effective control of foot-and-mouth disease (FMD) requires sensitive, specific and rapid diagnostic tools. However, the control and eradication of FMD in Africa is complicated by, among other factors, the existence of five of the seven FMD virus (FMDV) serotypes, including the SAT-serotypes 1, 2 and 3 that are gen...

journal_title：Journal of virological methods

authors： Chitray M,Grazioli S,Willems T,Tshabalala T,De Vleeschauwer A,Esterhuysen JJ,Brocchi E,De Clercq K,Maree FF

更新日期：2018-05-01 00:00:00

abstract：:Enteroviruses (EVs), such as the Coxsackie B-viruses (CVBs), are common human pathogens, which can cause severe diseases including meningitis, myocarditis and neonatal sepsis. EVs encode two proteases (2Apro and 3Cpro), which perform the proteolytic cleavage of the CVB polyprotein and also cleave host cell proteins to...

journal_title：Journal of virological methods

authors： Laitinen OH,Svedin E,Kapell S,Hankaniemi MM,Larsson PG,Domsgen E,Stone VM,Määttä JAE,Hyöty H,Hytönen VP,Flodström-Tullberg M

更新日期：2018-05-01 00:00:00

abstract：:Peach rosette mosaic disease was first described in the 1940s affecting peach and plum. It was later determined that peach rosette mosaic virus (PRMV) is the causal agent of the disease. PRMV, a member of the genus Nepovirus, infects several perennial crops including stone fruit, grape and blueberry as well as several...

journal_title：Journal of virological methods

authors： Ho T,Harris A,Katsiani A,Khadgi A,Schilder A,Tzanetakis IE

更新日期：2018-04-01 00:00:00

abstract：:Quantitative PCR (qPCR) assay using TaqMan™ probe was widely used in the detection of different nucleic acids. However, this technology has several drawbacks, including false negative results caused by primer-dimer (PD) and false positive issues due to primer-probe aggregations. Here, we designed a modified TaqMan™-Mo...

journal_title：Journal of virological methods

authors： Jiang W,Yue S,He S,Chen C,Liu S,Jiang H,Tong H,Liu X,Wang J,Zhang F,Sun H,Li M,Wang C

更新日期：2018-04-01 00:00:00

abstract：BACKGROUND:The HIV/HCV/HBsAg Triplex consists in manually performed, visually interpreted, lateral flow, immunochromatographic rapid diagnostic test simultaneously detecting in 15min human immunodeficiency virus (HIV)-1 and HIV-2 and hepatitis C virus (HCV)- specific antibodies (Ab) (IgG and IgM) and hepatitis B virus ...

journal_title：Journal of virological methods

authors： Robin L,Mboumba Bouassa RS,Nodjikouambaye ZA,Charmant L,Matta M,Simon S,Filali M,Mboup S,Bélec L

更新日期：2018-03-01 00:00:00

abstract：:Diagnosis of hepatitis C virus (HCV) infection is based on testing for antibodies to HCV (anti-HCV), hepatitis C core antigen (HCV cAg) and HCV RNA. To ensure quality control (QC) and quality assurance (QA), proficiency panels are provided by reference laboratories and various international organizations, requiring co...

journal_title：Journal of virological methods

authors： Poe A,Duong NT,Bedi K,Kodani M

更新日期：2018-03-01 00:00:00

abstract：:TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7 phages were quantified by TaqMan or SYBR Green qPCR referenced against M13 a...

journal_title：Journal of virological methods

authors： Peng X,Nguyen A,Ghosh D

更新日期：2018-02-01 00:00:00

abstract：:Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (AIVs). However, a successful surveillance regime requires proper treatment of samples in the field - rapid placement of samples in -80°C and subsequent maintenance of cold-chain. Given the logistical difficulties of t...

journal_title：Journal of virological methods

authors： Wille M,Yin H,Lundkvist Å,Xu J,Muradrasoli S,Järhult JD

更新日期：2018-02-01 00:00:00

abstract：:To formulate inactivated influenza vaccines, the concentration of hemagglutinin (HA) must be accurately determined. The standard test currently used to measure HA in influenza vaccines is the Single Radial Immunodiffusion (SRID) assay. We developed a very rapid, simple and sensitive alternative quantitative HA assay, ...

journal_title：Journal of virological methods

authors： Buffin S,Ikhelef N,Prudent J,Dubayle J,Nougarede N,Varenne MP,Moste C,Legastelois I

更新日期：2018-01-01 00:00:00

abstract：:Chronic HCV infection is one of the leading causes of liver-related death and in many countries it is a primary reason for having a liver transplant. HCV genotype identification has long been used in the clinical practice, since different genotypes have different response rates and required different doses and duratio...

journal_title：Journal of virological methods

authors： Dirani G,Paesini E,Mascetra E,Farabegoli P,Dalmo B,Bartolini B,Garbuglia AR,Capobianchi MR,Sambri V

更新日期：2018-01-01 00:00:00

abstract：:Sequence analysis of duck plague virus (DPV) revealed that there was a 528bp (B fragment) deletion within the UL2 gene of DPV attenuated vaccine strain in comparison with field virulent strains. The finding of gene deletion provides a potential differentiation test between DPV virulent strain and attenuated strain bas...

journal_title：Journal of virological methods

authors： Xie L,Xie Z,Huang L,Wang S,Huang J,Zhang Y,Zeng T,Luo S

更新日期：2017-11-01 00:00:00

abstract：:Development of a HIV-1 vaccine is a major global priority. The yellow fever virus (YFV) attenuated vaccine 17D is among the most effective of currently used vaccines. However, the stability of the YFV17D vector when carrying non-flavivirus genes has been problematic. We have constructed and expressed HIV-1 Env in YFV1...

journal_title：Journal of virological methods

authors： Yu JS,Liao HX,Pritchett J,Bowman C,Vivian C,Parks R,Xia SM,Cooper M,Williams WB,Bonsignori M,Reed SG,Chen M,Vandergrift N,Rice CM,Haynes BF

更新日期：2017-11-01 00:00:00

abstract：:This study aimed at assessing the specificity of the Elecsys® HIV combi PT in comparison to the ARCHITECT® HIV Ag/Ab Combo. With both of these assays, 3997 unselected sera from patients of a tertiary health care centre in Basel, Switzerland, were screened for HIV. Reactive sera were reanalysed on the VIDAS® HIV Duo Ul...

journal_title：Journal of virological methods

authors： Blaich A,Buser A,Stöckle M,Gehringer C,Hirsch HH,Battegay M,Klimkait T,Frei R

更新日期：2017-11-01 00:00:00

abstract：:Portable user-friendly diagnostic tests can benefit detection and surveillance of wildlife diseases. Here, the performance of a compact POCKIT™ Nucleic Acid Analyzer for detection of Ambystoma tigrinum virus (ATV), an emerging Iridovirus that is associated with high host mortality in the western tiger salamander (Amby...

journal_title：Journal of virological methods

authors： Lung O,Reimer SA,Goater CP

更新日期：2017-11-01 00:00:00

abstract：:A dot-immunobinding assay (DIBA) was optimized and used successfully for the rapid detection of 15 known viruses [Alfalfa mosaic virus (AMV), Bean pod mottle virus (BPMV), Bean yellow mosaic virus (BYMV), Cowpea mild mottle virus (CPMMV), Cowpea severe mosaic virus (CPSMV), Cucumber mosaic virus (CMV), Peanut mottle v...

journal_title：Journal of virological methods

authors： Ali A

更新日期：2017-11-01 00:00:00

abstract：:Human immunodeficiency virus (HIV) encodes a transcription trans-activator (Tat) with an essential role in the transcriptional elongation of viral RNA based on the viral promoter long terminal repeat (LTR). Tat-mediated transcription is conserved and can be distinguished from host transcription, so it is a therapeutic...

journal_title：Journal of virological methods

authors： Shin Y,Choi BS,Kim KC,Kang C,Kim K,Yoon CH

更新日期：2017-11-01 00:00:00

abstract：:A method for accurate quantitation of virus particles has long been sought, but a perfect method still eludes the scientific community. Electron Microscopy (EM) quantitation is a valuable technique because it provides direct morphology information and counts of all viral particles, whether or not they are infectious. ...

journal_title：Journal of virological methods

authors： Blancett CD,Fetterer DP,Koistinen KA,Morazzani EM,Monninger MK,Piper AE,Kuehl KA,Kearney BJ,Norris SL,Rossi CA,Glass PJ,Sun MG

更新日期：2017-10-01 00:00:00

abstract：:Virological monitoring through plasma viral load (PVL) quantification is essential for clinical management of HIV patients undergoing antiretroviral treatment (ART), and for detecting treatment failure. Quantitative PCR (qPCR)-based tests are the gold standard for measuring PVL. Largely because of their high cost, how...

journal_title：Journal of virological methods

authors： Boobalan J,Torti A,Dinesha TR,Solomon SS,Balakrishnan P,Saravanan S

更新日期：2017-10-01 00:00:00

abstract：:In this study, we aim to determine what effects prolonged storage and repeated freeze/thaw cycles have on the stability of influenza A(H1N1)pdm09 (influenza A/H1N1)RNA. Cloned influenza A/H1N1 RNA transcripts were serially diluted from 8.0-1.0 log10 copies/μl. RT-qPCR was used to measure RNA loss in transcripts stored...

journal_title：Journal of virological methods

authors： Granados A,Petrich A,McGeer A,Gubbay JB

更新日期：2017-09-01 00:00:00

abstract：:Studies were conducted to determine the performance of four dyes in assessing antiviral activities of compounds against three RNA viruses with differing cytopathogenic properties. Dyes included alamarBlue® measured by absorbance (ALB-A) and fluorescence (ALB-F), neutral red (NR), Viral ToxGlo™ (VTG), and WST-1. Viruse...

journal_title：Journal of virological methods

authors： Smee DF,Hurst BL,Evans WJ,Clyde N,Wright S,Peterson C,Jung KH,Day CW

更新日期：2017-08-01 00:00:00

abstract：BACKGROUND:Molecular assays for diagnosis of Flu A, Flu B, and RSV with short turn-around-time (TAT) are of considerable clinical importance. In addition, rapid and accurate diagnosis of a large panel of viral and atypical pathogens can be crucial in immunocompromised patients. OBJECTIVES:First, to evaluate the perfor...

journal_title：Journal of virological methods

authors： Steensels D,Reynders M,Descheemaeker P,Curran MD,Jacobs F,Denis O,Delforge ML,Montesinos I

更新日期：2017-07-01 00:00:00

abstract：:Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time....

journal_title：Journal of virological methods

authors： Mulholland C,McMenamy MJ,Hoffmann B,Earley B,Markey B,Cassidy J,Allan G,Welsh MD,McKillen J

更新日期：2017-07-01 00:00:00

abstract：:In light of the advances in HCV therapy, simplification of diagnosis confirmation, pre- treatment diagnostic workup and treatment monitoring is required to ensure broad access to interferon-free therapies. HCV core antigen (HCV cAg) testing is rapid, giving results in approximately 60min, and less expensive than HCV R...

journal_title：Journal of virological methods

authors： Rockstroh JK,Feld JJ,Chevaliez S,Cheng K,Wedemeyer H,Sarrazin C,Maasoumy B,Herman C,Hackett J Jr,Cohen DE,Dawson GJ,Cloherty G,Pawlotsky JM

更新日期：2017-07-01 00:00:00

abstract：:Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern I...

journal_title：Journal of virological methods

authors： Ahamed SF,Vivek R,Kotabagi S,Nayak K,Chandele A,Kaja MK,Shet A

更新日期：2017-06-01 00:00:00

abstract：:The occurrence of virus belonging to the putative genus Influenzavirus D, has been demonstrated all-around the world arousing interest within the scientific community. Most of the published virological surveys are based on the first described Real-Time PCR method, designed on the PB1 gene of the first isolate. The nec...

journal_title：Journal of virological methods

authors： Faccini S,De Mattia A,Chiapponi C,Barbieri I,Boniotti MB,Rosignoli C,Franzini G,Moreno A,Foni E,Nigrelli AD

更新日期：2017-05-01 00:00:00

abstract：:Despite intensive study, it is unclear which mechanisms are responsible for latent HIV infection in vivo. One potential mechanism is inhibition of HIV transcriptional elongation, which results in short abortive transcripts containing the trans-activation response (TAR) region. Because the relative levels of total (inc...

journal_title：Journal of virological methods

authors： Kaiser P,Joshi SK,Kim P,Li P,Liu H,Rice AP,Wong JK,Yukl SA

更新日期：2017-04-01 00:00:00

abstract：:Rice tungro disease (RTD) is a recurring disease affecting rice farming especially in the South and Southeast Asia. The disease is commonly diagnosed by visual observation of the symptoms on diseased plants in paddy fields and by polymerase chain reaction (PCR). However, visual observation is unreliable and PCR can be...

journal_title：Journal of virological methods

authors： Yee SF,Chu CH,Poili E,Sum MSH

更新日期：2017-02-01 00:00:00

abstract：:Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assa...

journal_title：Journal of virological methods

authors： Binkhamis K,Gillis H,Lafreniere JD,Hiebert J,Mendoza L,Pettipas J,Severini A,Hatchette TF,LeBlanc JJ

更新日期：2017-01-01 00:00:00

abstract：:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and preval...

journal_title：Journal of virological methods

authors： Yonemitsu K,Terada Y,Kuwata R,Nguyen D,Shiranaga N,Tono S,Matsukane T,Yokoyama M,Suzuki K,Shimoda H,Takano A,Muto M,Maeda K

更新日期：2016-12-01 00:00:00